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LUXFilm™ TEM Supports

Strong | Flat | Thin | Large Unobstructed Area | High Surface Attraction | Reduced Charging | No Autofluorescence

LUXFilm™ TEM supports were developed with assistance from the US National Institutes of Health (NIH). These strong, filmed supports can span very large unobstructed apertures – supplied standard up to 2mm ID. They are beam stable, highly transmissive, and robust to cryogenic temperatures. The supports improve the efficiency and throughput of TEM work by allowing the researcher to view the entire specimen; a significant improvement when looking at large structures, tracing features that appear over long distances in serial sections, searching for details that occur infrequently in the tissue sample, or when using specimen tilt for tomography.

FEATURES and BENEFITS...
LUXFilm™ is 5X stronger than Formvar. The supports are stable over the long beam exposures needed for tomography – without additional carbon coating. The films hold up to a variety of common stains* and to ethanol-based solutions.
LARGE UNOBSTRUCTED VIEWING WINDOWS – With a LUXFilm™ support, see your entire specimen without interference from grid wires.

FLAT – The films are stretched flat on the metal blank. Used in particle counting or screening applications, open area LUXFilm™ supports eliminate meniscus effects. Particles spread evenly, and do not collect next to the grid wires.
REDUCED CHARGING – The films exhibit very little drift or charging effects in the EM, compared with Formvar/carbon, improving imaging efficiency.

THIN – LUXFilm™ TEM supports are thin (50nm) and uniform. Most applications will not require additional carbon coating.

WETTABILITY – LUXFilm™ has a favorable inherent surface energy for epoxy-embedded sections. Serial ribbons “stick” and go down flat. Surface treatment can be performed to prepare the supports for negative stains and acrylic-embedded specimens.

NO AUTOFLUORESCENCE – LUXFilm™ exhibits no autofluorescence and no unspecific labeling with antibody stains, making it an ideal choice for correlative microscopy and immunocytochemistry in the EM. The large, unobstructed viewing windows assure that you will find all labeled features, even if rare.

 
Recommended Applications...

Demanding and routine TEM imaging

80-300 kV

Large sections

Tomography

Thick materials – films hold up to higher EM voltages

Pathology

Particle count and screening studies

Immunocytochemistry

CEMOVIS

tem filters

LUXFilm™  TEM support FAQs

Product Availability
When will LUXFilm TEM supports be available to purchase?
Can I make LUXFilm myself?
Can I get LUXFilm on gold or gilded grids?
Can I get LUXFilm with holes?
Can I get a custom thickness?
I use Synaptek (or other specialty) grids. Can I get LUXFilm on different grid blanks?
Product Performance
How does LUXFilm compare to SiN grids available commercially?
Is LUXFilm hydrophilic?
Is LUXFilm electrically conductive?
I want to do x-ray microanalysis of my specimen using LUXFilm. What chemical signals should I expect from the film?

Product Availability

When will LUXFilm TEM supports be available to purchase?

Limited quantities are available now; contact us to request price and availability of your desired product.

Can I make LUXFilm myself?

No. LUXFilm is made through a proprietary process.

Can I get LUXFilm on gold or gilded grids?

Yes, but gold or gilded grids are not presently part of the LUXFilm line. For a support for immunogold, other immune-labeling protocols, or cell culture, please try our standard nickel grids. For gold, contact our sales department for a custom quote.

Can I get LUXFilm with holes?

No. Holey LUXFilm is not offered at this time.

Can I get a custom thickness?

Yes, depending on the grid style, LUXFilm can be made thinner or thicker to order. There will be a minimum order required. Contact our sales department for a custom quote.

I use Synaptek (or other specialty) grids. Can I get LUXFilm on different grid blanks?

Yes, and no. The LUXFilm grid blanks that are offered provide stiffness equivalent to many commercial grids. You should find them easy to handle. However, our process does not accommodate specialty grids with handles or clamshells. These would have to be quoted on an individual basis. Contact our sales department for a custom quote.

 

Product Performance

How does LUXFilm compare to SiN grids available commercially?

The 2mm LUXFilm open aperture support is larger than any SiN membrane window presently offered. LUXFilm is a polymeric film, so its chemical composition differs from SiN. LUXFilm contains C-H-O and N

Is LUXFilm hydrophilic?

Yes, most users have found that plastic sections, 2D crystallized membrane proteins, and many other particles adhere very well to LUXFilm. Common treatments such as Alsatian blue, mild detergent, and glow discharge may be used prior to negative staining. Cryosections may still require mechanical attachment, so LUXFilm should be ordered in a gridded or parallel bar configuration.  Luxel can also perform additional surface treatment to increase wetting.  Please contact us to learn more.

Is LUXFilm electrically conductive?

The films are not conductive per se, however, user input has told us they do not display charging effects in use. If you require carbon coating for conductivity, the films will stand up to this – but, we’d ask you try some as they are first.

I want to do x-ray microanalysis of my specimen using LUXFilm. What chemical signals should I expect from the film?

LUXFilm contains only C, H, O, and N. The films are fabricated and the supports are assembled in a clean-controlled facility.

 

 

 

 

 

 

 

 

 

   
P.O. Box 1879 (Mail)   515 Tucker Ave (Parcels)                   Friday Harbor, WA  98250  USA                    Tel: 360-378-4137         Fax: 360-378-4266
luxel@luxel.com